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Related Experiment Video

Updated: Jun 8, 2026

Microfluidics in Assessing Platelet Function
06:47

Microfluidics in Assessing Platelet Function

Published on: November 8, 2024

Do we need time adjusted mean platelet volume measurements?

Marcus D Lancé1, Rene van Oerle, Yvonne M C Henskens

  • 1Deptartment of Anaesthesiology and Pain Treatment, Maastricht University Medical Center, Maastricht, the Netherlands. marcus.lance@mumc.nl

Laboratory Hematology : Official Publication of the International Society for Laboratory Hematology
|September 23, 2010
PubMed
Summary

Standardizing mean platelet volume (MPV) measurement is crucial. MPV is smaller in citrate tubes than EDTA tubes, with optimal stability at 120 minutes post-collection.

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Area of Science:

  • Hematology
  • Clinical Laboratory Science

Background:

  • Mean platelet volume (MPV) is a significant indicator linked to various disease states.
  • Existing research highlights anticoagulant and time-dependent variations in MPV.
  • Standardized laboratory methods are essential for accurate MPV assessment.

Purpose of the Study:

  • To standardize the measurement of mean platelet volume (MPV).
  • To establish a reliable reference range for MPV.
  • To investigate the impact of different anticoagulants and time on MPV.

Main Methods:

  • Blood samples were collected in potassium-ethylenediaminetetra-acid (EDTA) and sodium-citrate tubes from healthy volunteers.
  • MPV and platelet count were measured at 30-minute intervals for 4 hours.
  • Measurements were performed at the point of highest stability to determine a reference range.

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Last Updated: Jun 8, 2026

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Main Results:

  • Citrate samples showed significantly lower MPV (7.0 fL ± 0.69 SD) compared to EDTA samples (8.0 fL ± 0.8 SD).
  • Platelet swelling occurred up to 120 minutes in EDTA and 60 minutes in citrate.
  • Platelet count stability was highest in EDTA; no inverse MPV-platelet count correlation was observed in the second group.

Conclusions:

  • Platelet volume is significantly influenced by the anticoagulant used, with citrate tubes yielding smaller MPV values.
  • Optimal timing for MPV measurement is 120 minutes post-venipuncture to ensure stability.
  • Established reference ranges for MPV differ between EDTA (7.2-10.8 fL) and citrate (6.1-9.5 fL) tubes.