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Related Concept Videos

NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
Applications Of NMR In Biology01:25

Applications Of NMR In Biology

Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
The...
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other axis.
¹H NMR Signal Integration: Overview00:58

¹H NMR Signal Integration: Overview

The intensity of a signal, which can be represented by the area under the peak, depends on the number of protons contributing to that signal. The area under each peak is shown as a vertical line called an integral, with the integral value listed under it, as seen in the proton NMR spectrum of benzyl acetate. Each integral value is divided by the smallest integral value to obtain the ratio of the number of protons producing each signal. The ratio reveals the relative number of protons and not...
Sample Preparation for Analysis: Advanced Techniques01:08

Sample Preparation for Analysis: Advanced Techniques

Accurate analysis of complex samples often requires advanced preparation techniques to achieve reliable and reproducible results. Samples containing inorganic or organic materials can be challenging to dissolve or decompose effectively. Standard sample preparation methods include acid digestion, fusion, dry ashing, and wet digestion.
Acid digestion with strong acids is commonly used to dissolve inorganic materials that are insoluble (do not dissolve) in water. This method can be useful for...

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Updated: Jun 8, 2026

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the &#181;s-ms Timescale
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15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

Published on: April 19, 2021

Gifa V. 4: A complete package for NMR data set processing.

J L Pons1, T E Malliavin, M A Delsuc

  • 1Centre de Biochimie Structurale, INSERM U414, CNRS UMR 9955, UniversitĂ© Montpellier 1, FacultĂ© de Pharmacie, 15 Avenue Charles Flahault, F-34060, Montpellier, France.

Journal of Biomolecular NMR
|September 23, 2010
PubMed
Summary
This summary is machine-generated.

Gifa is a versatile NMR data processing software. It offers modular processing, analysis, and display for 1D, 2D, and 3D NMR datasets, suitable for all user levels.

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Area of Science:

  • Chemistry
  • Biochemistry
  • Structural Biology

Background:

  • Nuclear Magnetic Resonance (NMR) spectroscopy is a powerful technique for determining molecular structure and dynamics.
  • Processing and analyzing NMR data can be complex, requiring specialized software.
  • Existing NMR software may lack versatility or user-friendliness for diverse applications.

Purpose of the Study:

  • To introduce Gifa, a novel software program for processing, displaying, and analyzing NMR data.
  • To highlight the modular design and versatility of Gifa for various NMR applications.
  • To cater to both novice and experienced users in the field of NMR spectroscopy.

Main Methods:

  • Gifa is built with a modular architecture comprising three independent modules.
  • Module 1: Basic processing operations (apodization, Fourier Transforms, phasing, baseline correction, peak picking, line fitting, linear prediction, maximum entropy).
  • Module 2: Command language primitives for complex macro commands. Module 3: Graphic commands for building a user interface.

Main Results:

  • Gifa provides comprehensive tools for 1D, 2D, and 3D NMR data analysis.
  • The modular design allows for easy extension and customization based on user needs.
  • The program supports interactive and batch modes on UNIX systems, with or without a graphic display.

Conclusions:

  • Gifa offers a versatile and user-friendly solution for NMR data processing and analysis.
  • Its modularity and extensibility make it adaptable to a wide range of research requirements.
  • Gifa is suitable for both beginners and advanced users in NMR spectroscopy.