[Population genetics of 11 STR loci in the Upper Silesia population]

The report present the results of population studies of 11 STR loci: D16S5539, D7S820, D13S317, CSF1PO, TPOX, TH01, F13A01, FESFPS, VWA, F13B, LPL originating from samples examined in Laboratory of Biological traces, Chair and Department of Forensic Medicine, Silesian Medical University. The examinations were performed in the group of 455 non-consanguinated adults, men and women, from Upper Silesia. The purpose of the investigation was to study the distribution of allele frequencies of 11 short tandem repeat representative sample of the Upper Silesia (Poland) population, to show genetic balance in agreement with Hardy-Weinberg's law for the studied population and to compare homogeneity of the usefulness of 11 STR genetic markers for paternity testing and forensic identification purposes. Genomic DNA was isolated from blood samples and swabs from oral cavity by The Blood DNA Prep Plus kit (A&A Biotechnology) and EZ1 DNA tissue Kit for the use on the BioRobot EZ1. All reactions were carried out according to the manufacturer's recommendations (Silver Stain Detection, Promega). POR amplification was performed following the instructions of the GenePrint STR System kit using a GeneAmp PCR System 2700 Thermal Cykler. PCR products and Promega Ladders were separated by vertical electrophoresis on 6% denaturing polyacrilamide gel (Amresco), electrophoresis and visualized by silver staining (Promega Corporation). Allele frequencies, the values of heterozygosity (Ht), polymorphism information content (PIC), power of discrimination(PD), power of exclusion (PE), paternity index (PI), and matching probability (PM) were calculated using FatRec by Dudek's program and TFPGA of Miller. The concordance with HWE was evaluated using the chi-square test. The compared statistic parameters, which make an important component of research work and opinionating, show that 11 STR loci constitutes a very useful tool for individual identification in crime research and in putative paternity research.