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Updated: Jun 8, 2026
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Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
Published on: November 27, 2017
Isolation and characterization of equine amniotic fluid-derived multipotent stem cells
Sang-Bum Park1, Min-Soo Seo, Jun-Gu Kang
1Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
Cytotherapy
|September 24, 2010
Summary
Equine amniotic fluid (AF) yields multipotent stem cells (MSC) with self-renewal and differentiation abilities. These equine AF-derived MSC (eAF-MSC) show promise for cell therapy applications in horses.
Area of Science:
- Veterinary Medicine
- Stem Cell Biology
- Regenerative Medicine
Background:
- Amniotic fluid (AF) is a known source of stem cells.
- No prior reports existed on equine AF stem cells.
- This study aimed to isolate and characterize equine AF-derived stem cells.
Purpose of the Study:
- To isolate multipotent stem cells (MSC) from equine amniotic fluid (eAF-MSC).
- To assess the self-renewal capacity of eAF-MSC.
- To evaluate the multilineage differentiation potential of eAF-MSC.
Main Methods:
- Equine AF was collected from thoroughbred mares.
- Mononuclear cells (MNC) were isolated using Ficoll-Paque density gradient.
- Immunophenotype was characterized by flow cytometry, and trilineage differentiation assays were performed.
Main Results:
- Equine AF-derived multipotent stem cells (eAF-MSC) were successfully isolated.
- eAF-MSC demonstrated high proliferation levels and self-renewal ability.
- Flow cytometry confirmed typical MSC phenotypic markers, and differentiation assays showed trilineage capability.
Conclusions:
- Equine amniotic fluid is a viable source for isolating multipotent stem cells.
- Equine AF-derived MSC (eAF-MSC) possess characteristics suitable for cell therapy.
- These findings suggest potential applications for eAF-MSC in equine regenerative medicine.
