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Constant Pressure-controlled Extrusion Method for the Preparation of Nano-sized Lipid Vesicles
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Constant Pressure-controlled Extrusion Method for the Preparation of Nano-sized Lipid Vesicles

Published on: June 22, 2012

A new method for liposome preparation using a membrane contactor.

Chiraz Jaafar-Maalej1, Catherine Charcosset, Hatem Fessi

  • 1Université de Lyon, Lyon, France.

Journal of Liposome Research
|September 24, 2010
PubMed
Summary

A new membrane contactor technique enables scalable liposome preparation for pharmaceutical agents. This robust method ensures high drug entrapment efficiency and stable, nanometric liposomes.

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Preparation, Purification, and Use of Fatty Acid-containing Liposomes
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Preparation, Purification, and Use of Fatty Acid-containing Liposomes

Published on: February 9, 2018

Area of Science:

  • Pharmaceutical Technology
  • Colloid Science
  • Biomaterials Engineering

Background:

  • Liposomes are crucial drug delivery vehicles.
  • Efficient and scalable liposome preparation remains a challenge.
  • Current methods often lack reproducibility and control over vesicle characteristics.

Purpose of the Study:

  • To introduce a novel, scalable liposomal preparation technique using a membrane contactor.
  • To investigate the influence of key process parameters on liposome characteristics.
  • To evaluate the drug entrapment efficiency and stability of liposomes prepared by this method.

Main Methods:

  • Adaptation of the ethanol-injection technique with a specialized membrane contactor module.
  • Systematic study of parameters including organic-phase pressure, aqueous-phase flow rate, and phospholipid concentration.
  • Preparation and characterization of drug-loaded liposomes using hydrophobic drug models (indomethacin, beclomethasone dipropionate).
  • Analysis of vesicle size distribution, morphology (Transmission Electron Microscopy), entrapment efficiency, and long-term stability.

Main Results:

  • Vesicle size distribution was successfully controlled by adjusting organic-phase pressure, aqueous-phase flow rate, and phospholipid concentration.
  • The membrane contactor technique demonstrated robustness, reproducibility, and long-term stability of lipid vesicles.
  • High entrapment efficiencies were achieved for indomethacin (63%) and beclomethasone dipropionate (98%).
  • Transmission electron microscopy confirmed the formation of nanometric, quasispherical multilamellar vesicles (50–160 nm).

Conclusions:

  • The membrane contactor-based ethanol-injection technique offers a scalable and robust method for liposome preparation.
  • This technique allows for precise control over liposome size and high drug encapsulation efficiency.
  • It holds significant potential for the industrial production of liposomal pharmaceutical formulations.