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Related Experiment Video

Updated: Jun 8, 2026

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Published on: January 31, 2025

Plasma membrane helps autophagosomes grow.

Brinda Ravikumar¹, Kevin Moreau, David C Rubinsztein

¹Department of Medical Genetics, Cambridge, UK.

|September 24, 2010

Summary

The plasma membrane contributes to autophagosome formation through clathrin-mediated endocytosis. This process involves Atg16L1 interacting with clathrin, impacting autophagosome biogenesis.

Area of Science:

Cell Biology
Autophagy Research
Membrane Trafficking

Background:

The origin of autophagosomes, crucial for cellular recycling, remains incompletely understood.
Multiple membrane sources have been proposed for autophagosome biogenesis.

Purpose of the Study:

To investigate the role of the plasma membrane in autophagosome formation.
To elucidate the involvement of clathrin-mediated endocytosis in autophagosome biogenesis.

Main Methods:

Live-cell imaging to track Atg16L1-positive structures.
Investigating interactions between Atg16L1, clathrin heavy-chain, and AP2.
Utilizing gene knockdowns to assess the impact on autophagosome formation.

Main Results:

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Last Updated: Jun 8, 2026

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

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Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond

Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond

Published on: July 27, 2013

Imaging ATG9A, a Multi-Spanning Membrane Protein

Imaging ATG9A, a Multi-Spanning Membrane Protein

Published on: June 16, 2023

The plasma membrane contributes to pre-autophagic structures via clathrin-mediated endocytosis.
Atg16L1 localizes to clathrin- and cholera toxin B-positive vesicles near the plasma membrane.
Disruption of clathrin-mediated endocytosis impairs Atg16L1-positive structure formation and autophagosome biogenesis.

Conclusions:

The plasma membrane is a source for autophagosome formation.
Clathrin-mediated endocytosis plays a key role in initiating autophagosome biogenesis.
Atg16L1 is involved in linking plasma membrane-derived vesicles to autophagosome formation.