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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 8, 2026

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells
09:20

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

Published on: August 11, 2020

Fiber-optic laser scanning confocal microscope suitable for fluorescence imaging.

P M Delaney, M R Harris, R G King

    Applied Optics
    |September 24, 2010
    PubMed
    Summary
    This summary is machine-generated.

    A novel fiber-optic laser scanning confocal microscope offers comparable performance to traditional systems. Its compact design enhances flexibility and portability for advanced imaging applications.

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    Related Experiment Videos

    Last Updated: Jun 8, 2026

    Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells
    09:20

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    Published on: August 11, 2020

    Imaging Subcellular Structures in the Living Zebrafish Embryo
    11:19

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    Published on: April 2, 2016

    Video-rate Scanning Confocal Microscopy and Microendoscopy
    14:10

    Video-rate Scanning Confocal Microscopy and Microendoscopy

    Published on: October 20, 2011

    Area of Science:

    • Optical microscopy
    • Confocal microscopy
    • Fiber optics

    Background:

    • Conventional laser scanning confocal microscopes (LSCMs) utilize bulk optics.
    • Existing LSCMs can be bulky, complex, and limited in portability.
    • There is a need for more flexible and compact confocal imaging solutions.

    Purpose of the Study:

    • To describe a fiber-optic laser scanning confocal microscope.
    • To present a compact and portable scanning head design.
    • To evaluate the performance of this novel confocal microscope.

    Main Methods:

    • Utilized a single-mode optical fiber as both the light source and detection aperture.
    • Designed a compact, portable scanning head compatible with standard light microscopes.
    • Tested resolution and imaging in reflectance and fluorescence modes with various objective lenses.

    Main Results:

    • Achieved performance comparable to conventional bulk optical LSCMs.
    • Demonstrated robust alignment and ease of integration with standard microscopes.
    • Showcased effective imaging capabilities in both reflectance and fluorescence modes.

    Conclusions:

    • The developed fiber-optic confocal microscope provides a viable alternative to conventional systems.
    • Its compact and portable design offers enhanced flexibility for diverse imaging tasks.
    • This technology enables confocal microscopy in applications previously restricted by instrument limitations.