Related Experiment Video
Updated: Jun 8, 2026

Clarifying and Imaging Candida albicans Biofilms
Published on: March 6, 2020
Influence of spherical aberration on axial imaging of confocal reflection microscopy
Abstract:
The influence of spherical aberration on axial imaging of confocal reflection microscopy is investigated. In particular, the effects of lens aperture size and of the first three orders of spherical aberration are inspected. It is shown both theoretically and experimentally that the aberrated axial response can be improved by slightly reducing the lens aperture size. The experimental results concerning the effect of the tube length on the axial response and the aberration compensation are also given.
Related Concept Videos
Confocal Fluorescence Microscopy
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Total Internal Reflection Fluorescence Microscopy
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

