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Updated: Jun 8, 2026

Measuring Volatile and Non-volatile Antifungal Activity of Biocontrol Products
Published on: December 5, 2020
[Safe use of carbendazim in GAP of Atractylodes macrocephala]
Jian Lin1, Houdao Wei, Tianyu Wang
1Pesticide Environmental Toxicology Research Institute, Zhejiang University, Hangzhou 310029, China.
Objective:
To study the residue of in roots of Atractylodes macrocephalal and in soil.
Method:
Samples were extracted with methanol. The extracts were cleaned up by liquid-liquid extraction and detected by HPLC.
Result:
Repeatability and accuracy of the method was verified by fortified recovery at 0.01, 0.05, 0.1, 0.2 mg x kg(-1) levels. Average recovery were 86.1%-98.3% and RSD were 1.0%-6.5% in root and soil. A. macrocephala was treated with two dosage of carbendazim during growing. Results of field test showed that the half lives of carbendazim were 6.51-7.98 d in cultivated soil, 4.51-6.50 d in roots, separately. After sample was preliminarily processed, the residue of dried samples was 0.042-0.433 mg x kg(-1), higher than the fresh samples.
Conclusion:
If 0.2 mg x kg(-1) is recommended as the MRL (maximum residues limited) of carbendazim in the roots of A. macrocephala, it is suggested that the dose of 0.675 kg a.i. x hm(-1) carbendazim is sprayed twice a year, and carbendazim should not be used within 21 days before the harvest.
