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[Study on antioxidant effect of Herba Lycopi and screening of active fractions]
1Key Laboratory of Chinese Internal Medicine Ministry of Education and Beijing City, Dongzhimen Hospital Affiliated to Bejing University of Chinese Medicine, Beijing 100700, China. nieboww_1977@163.com
Objective:
To Study the antioxidant activity of Herba Lycopi, and screen the main active fractions.
Method:
The different polar fractions were isolated from the ethanol extract of Herba Lycopi. The scavenging activity to DPPH and superoxide anions, reducing effect on Fe3+, and inhibiting activity of lipid peroxidation of the fractions were studied in vitro by high-throughput screening technique.
Result:
The ethanol extract, ethyl acetate fraction (LLE-A. E, MCCM2921) and n-buthanol fraction (LLE-A. B, MCCM2922) of Herba Lycopi showed the scavenging activity to DPPH and superoxide anions, reducing effect on Fe3+, and inhibiting activity of lipid peroxidation.
Conclusion:
The ethanol extract and different polar fractions showed antioxidant capacity in vitro. That may be explain that the effect of activating blood and dissolving stasis and anti-fibrosis of Herba Lycopi in traditional application. The results may be helpful for further development of Herba Lycopi as a low-toxic antioxidant drug.