Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The effect of rapid improvement of blood glucose level on diabetic neuropathy in Korean people with diabetes mellitus.

Diabetes research and clinical practice·2026
Same author

Synergistic dual doping and directed structural engineering of 2D cobalt tellurium nanoarchitectures for practical-level overall water splitting.

Materials horizons·2026
Same author

Nanoparticle-enriched mass spectrometry enables comprehensive plasma proteomics and reveals novel candidates for mild cognitive impairment.

Scientific reports·2026
Same author

Hypoxia-Induced Fibroblast IL-6 Promotes Immunosuppressive Macrophage Phenotypes in Pancreatic Cancer.

Cells·2026
Same author

[Development of a Computer-Aided Automatic-Detection Deep-Learning Algorithm to Identify a Urinary Stone in Low-Dose Non-Enhanced CT Images].

Journal of the Korean Society of Radiology·2026
Same author

Epidemiology of sepsis in emergency departments: insights from the National Emergency Department Information System (NEDIS) database in Korea, 2020-2024.

Clinical and experimental emergency medicine·2026

Related Experiment Video

Updated: Jun 8, 2026

Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
10:43

Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

Aptamer-mediated surface-enhanced Raman spectroscopy intensity amplification.

Nam Hoon Kim1, Seung Joon Lee, Martin Moskovits

  • 1Department of Chemistry and Biochemistry, University of California, Santa Barbara, California 93106-9510, United States.

Nano Letters
|September 25, 2010
PubMed
Summary
This summary is machine-generated.

This study developed a novel aptasensor for adenosine detection. It utilizes a DNA aptamer to amplify surface-enhanced Raman spectroscopy signals, enabling sensitive and specific detection of adenosine molecules.

More Related Videos

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

Related Experiment Videos

Last Updated: Jun 8, 2026

Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
10:43

Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging
09:46

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Published on: April 28, 2022

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Surface-enhanced Raman spectroscopy (SERS) offers high sensitivity for molecular detection.
  • Aptamers are short DNA or RNA sequences that can bind to specific targets.
  • Controlling SERS hotspots is crucial for enhancing detection limits.

Purpose of the Study:

  • To develop a bifunctional DNA aptasensor for adenosine detection.
  • To create and control a SERS hotspot using an adenosine-sensitive aptamer.
  • To investigate the amplification mechanism of SERS signals by the aptamer.

Main Methods:

  • A bifunctional DNA aptamer sensitive to adenosine was designed.
  • A SERS hotspot was engineered between a bulk gold surface and a gold nanoparticle (Au NP).
  • The Au NP was functionalized with 4-aminobenzenethiol (4-ABT) as a Raman reporter.
  • Atomic force microscopy (AFM) was used for in situ imaging.

Main Results:

  • Adenosine binding induced a concentration-dependent increase in 4-ABT SERS intensity by up to ~4-fold.
  • AFM imaging revealed a ~5 nm decrease in aptamer-Au NP height upon adenosine binding.
  • The aptamer's conformational change amplified the SERS signal from multiple reporter molecules.

Conclusions:

  • The developed aptasensor effectively detects adenosine by modulating a SERS hotspot.
  • The aptamer's molecular recognition event leads to significant SERS signal amplification.
  • This approach demonstrates a promising strategy for ultrasensitive biosensing applications.