Antioxidant flavone glycosides from the leaves of Fargesia robusta

The aqueous methanolic leaf extract of Fargesia robusta var. Pingwu was evaluated in vitro for its antioxidant capacity using the TEAC and ORAC assays. C-Glycosyl flavones, farobin A (1) and farobin B (2), together with three known compounds, tricin-5-O-glucopyranoside (3), 2''-O-α-rhamnosyl-6-C-(6-deoxy-ribo-hexos-3-ulosyl)luteolin (4), and luteolin-6-C-glucopyranoside (homoorientin) (5), were isolated from the hydroalcoholic extract of the leaves of F. robusta. The structures of the compounds were determined by spectroscopic analyses including UV, 1D and 2D NMR, and MS. Compounds 1, 4, and 5 exhibited potent antioxidant activity in the TEAC assay, while compounds 1, 3, and 5 showed the highest antioxidant capacity in the ORAC assay.