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Isolating Mesangiogenic Progenitor Cells (MPCs) from Human Bone Marrow
09:53

Isolating Mesangiogenic Progenitor Cells (MPCs) from Human Bone Marrow

Published on: July 15, 2016

Potential role of bone marrow-derived cells in the turnover of mesothelium

Kuo-Su Chen1, Chao-Hung Wang, Tzung-Hai Yen

  • 1Division of Nephrology, Chang Gung Memorial Hospital, Keelung, Taiwan. cksdavid@adm.cgmh.org.tw

Renal Failure
|September 25, 2010
PubMed
Abstract

Insights

Bone marrow cells can transform into mesothelium, the lining of the abdominal cavity. This study confirms bone marrow cells can integrate into and express markers of mesothelium in mice.

Area of Science:

  • Regenerative Medicine
  • Cell Biology
  • Transplantation Immunology

Background:

  • The origin of mesothelium, the serous membrane lining body cavities, remains incompletely understood.
  • Bone marrow cells have been hypothesized as a potential source for mesothelial regeneration, but direct evidence is limited.

Purpose of the Study:

  • To investigate the potential of bone marrow-derived cells to contribute to mesothelium formation.
  • To track donor bone marrow cells within the peritoneal membrane of recipient mice.

Main Methods:

  • Cross-gender and green fluorescent protein (GFP)-mismatched bone marrow transplantation was performed in mice.
  • Peritoneal tissues were analyzed using immunohistochemistry (IHC) and chromogenic in situ hybridization (CISH) to detect donor cells.
  • Analysis included detection of GFP and Y-chromosome markers at various time points post-transplantation.

Main Results:

  • Donor bone marrow cells (GFP+) were identified in the peritoneal membrane of recipients, albeit at low engraftment levels (0.7%-2.2%).
  • Y-chromosome detection confirmed the presence of donor marrow cells within the mesothelium.
  • Incorporated donor cells expressed cytokeratin, a mesothelial marker.

Conclusions:

  • Bone marrow-derived cells can integrate into the mesothelium in bone marrow transplant recipients.
  • These integrated cells exhibit mesothelial characteristics, suggesting a role in mesothelial turnover and repair.

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