[Effects of extraneous wild type p53 on malignant growth of human lung cancer cell line.]

To study the effects of extraneous wild type p53 on malignant phenotype of human lung cancer cell line (801-D) with mutant type p53 gene.

The human lung cancer line (801-D) with mutant type p53 was selected and characterized with PCR-SSCP and DNA sequence analysis. Recombinant plasmid PZiPp53 was constructed , which expressed wild type p53. A transfected cell line , 801-D-p53 was obtained after transfering the plasmid into 801-D cell line by gene gun mediated and selected by G418. The wild type p53 and the alteration of malignant phenotype were detected in the transfected cell line by PCR method and cell line culture and heteroplastic transplant study.

The extraneous wild type p53 gene was detected in 801-D-p53 cell line cultured in vitro one year after transfection. The growth of the transfected cell line was inhibited comparing the parent cell line. The colony formation inhibition rate of the cell line was 96% and the tumorigenicity in nude mice was suppressed. The tumor growth significantly slow and the average volume of the transplant tumor was 1/28 of that of the parent cell line.

Extraneous wild type p53 gene may stably exist in the human lung cancer cell line with mutant type p53 after wild type p53 transfection and suppress the malignant phenotype of the transfected cell line. These results indicate that the recombinant plasmid expressing wild type p53 may be useful for gene therapy of human lung cancer.