Enzyme entrapment by β-cyclodextrin electropolymerization onto a carbon nanotubes-modified screen-printed electrode

Area of Science:

• Electrochemistry
• Biosensor Technology
• Nanomaterials

Background:

• Electropolymerization offers a versatile method for creating modified electrodes.
• Enzyme immobilization is crucial for biosensor development and stability.
• Multi-walled carbon nanotubes (MWCNT) enhance electrochemical properties.

Purpose of the Study:

• To develop a novel enzyme entrapment strategy using electropolymerization.
• To create a sensitive and specific biosensor for dopamine (DA) quantification.
• To evaluate the performance of the developed biosensor compared to existing methods.

Main Methods:

• Electropolymerization of β-cyclodextrin (β-CD) and glucose oxidase (GOx) onto screen-printed electrodes (SPE) modified with MWCNT.
• Fabrication and testing of various electrode configurations (SPE, SPE/β-CD, SPE/GOx, SPE/MWCNT/β-CD-GOx).
• Electrochemical analysis of dopamine in the presence of interfering agents (ascorbic acid, uric acid).

Main Results:

• The SPE/MWCNT/β-CD-GOx electrode demonstrated superior analytical performance compared to electrodes without MWCNT.
• The biosensor exhibited good reproducibility, repeatability, and an extended lifetime under cold storage.
• A low limit of detection (LOD) of 0.48±0.02 μA in a linear range of 10-50 μM was achieved with high sensitivity.
• Accurate DA quantification was possible even in the presence of common interfering substances.

Conclusions:

• The developed electropolymerization-based enzyme entrapment approach is effective for creating stable and sensitive biosensors.
• The inclusion of MWCNT significantly improves the biosensor's analytical characteristics.
• This method shows promise for applications requiring enhanced sensitivity, stability, and rapid response in biosensing systems.