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Related Experiment Video

Updated: Jun 8, 2026

Establishment of an Embryo Implantation Model In Vitro

Establishment of an Embryo Implantation Model In Vitro

Published on: June 21, 2024

Blastocyst gene expression correlates with implantation potential.

Jason C Parks¹, Blair R McCallie, Ann M Janesch

¹Colorado Foundation for Fertility Research, Lone Tree, Colorado, USA.

Fertility and Sterility

|September 25, 2010

Summary

Blastocyst gene expression in mice impacts implantation success and pregnancy loss. Specific gene changes, like decreased Eomes, predict implantation failure or absorption, offering insights into early pregnancy outcomes.

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Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Published on: January 30, 2017

Area of Science:

Reproductive biology
Developmental biology
Genetics

Background:

Implantation failure is a significant challenge in reproductive medicine.
The blastocyst stage is critical for successful pregnancy establishment.
Understanding molecular factors influencing blastocyst development and implantation is crucial.

Purpose of the Study:

To investigate the role of blastocyst gene expression in implantation failure.
To correlate trophectoderm (TE) gene expression with pregnancy outcomes in a mouse model.

Main Methods:

Retrospective study involving BDF1 female mice.
Trophectoderm biopsy of hatching blastocysts before single blastocyst transfer.
Quantitative real-time polymerase chain reaction (Q RT-PCR) for gene expression analysis of TE cells.
Transcriptome analysis of placental and absorption sites at day 16.

Main Results:

Decreased B3gnt5 and Eomes gene expression in blastocysts that failed to implant.
Decreased Wnt3a and Eomes gene expression in blastocysts resulting in spontaneous pregnancy loss (absorption).
Distinct gene expression signatures and altered complement/coagulation pathways in spontaneous pregnancy loss.

Conclusions:

Individual blastocyst gene expression profiles correlate with pregnancy outcomes.
TE gene expression is a potential biomarker for predicting implantation success and pregnancy loss.
This study provides novel insights into the molecular mechanisms underlying early pregnancy failure.