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Genomic characterization of Campylobacter jejuni strain M1

Carsten Friis1, Trudy M Wassenaar, Muhammad A Javed

  • 1Department of Systems Biology, The Technical University of Denmark, Lyngby, Denmark. carsten@cbs.dtu.dk

Plos One
|September 25, 2010
PubMed

Insights

This study sequenced the Campylobacter jejuni M1 genome, revealing its close relationship to strain 81116. The analysis identified the Campylobacter jejuni pan-genome and core genome, offering insights into strain diversity and virulence.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Campylobacter jejuni is a leading cause of bacterial gastroenteritis worldwide.
  • Direct transmission from animals to humans, though common for C. jejuni, is rarely documented for specific strains like M1.
  • Understanding strain-specific genomic features is crucial for public health interventions.

Purpose of the Study:

  • To sequence the genome of Campylobacter jejuni strain M1, a rare case of direct chicken-to-human transmission.
  • To compare strain M1's genome with other publicly available C. jejuni genomes.
  • To define the pan-genome, core genome, and accessory genes of C. jejuni, focusing on strain M1 and its closest relative, strain 81116.

Main Methods:

  • Whole-genome sequencing of Campylobacter jejuni strain M1.
  • Comparative genomics utilizing 13 C. jejuni genome sequences.
  • Bioinformatic analysis including pan-genome and core genome identification.
  • Phylogenetic analysis using 16S rRNA and gene family data.

Main Results:

  • The genome of C. jejuni strain M1 was successfully sequenced and found to be most similar to strain 81116.
  • The C. jejuni pan-genome was characterized, comprising 2,427 gene families.
  • The core genome consisted of 1,295 gene families, representing approximately two-thirds of the average C. jejuni genome content.
  • Unique genes between strains M1 and 81116 were identified, alongside auxiliary genes.

Conclusions:

  • The genomic analysis provides a foundation for understanding the genetic basis of C. jejuni strain M1's virulence.
  • Comparative genomics reveals significant insights into C. jejuni population structure and gene content variation.
  • This research contributes to the broader understanding of Campylobacter jejuni genomics and its implications for food safety and public health.

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