Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 8, 2026

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography
06:19

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

Published on: March 10, 2023

Design and implementation of high throughput screening assays.

Ricardo Macarrón1, Robert P Hertzberg

  • 1Molecular Discovery Research, GlaxoSmithKline, 1250 S Collegeville Road, Collegeville, PA 19426, USA. Ricardo.macarron@gsk.com

Molecular Biotechnology
|September 25, 2010
PubMed
Summary

High throughput screening (HTS) is crucial for drug discovery. Successful HTS assay design requires careful planning, interdisciplinary collaboration, and pragmatic decision-making to identify effective lead compounds.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparison of the enzymatic and cellular profiles of clinical JAK inhibitors for the treatment of myelofibrosis.

Blood neoplasia·2026
Same author

Selective targeting of mutated calreticulin by the monoclonal antibody INCA033989 inhibits oncogenic function of MPN.

Blood·2024
Same author

Discovery of (4-Pyrazolyl)-2-aminopyrimidines as Potent and Selective Inhibitors of Cyclin-Dependent Kinase 2.

Journal of medicinal chemistry·2024
Same author

Highly efficient genome editing in primary human bronchial epithelial cells differentiated at air-liquid interface.

The European respiratory journal·2020
Same author

Nuisance Compounds, PAINS Filters, and Dark Chemical Matter in the GSK HTS Collection.

SLAS discovery : advancing life sciences R & D·2018
Same author

Applications of CRISPR genome editing technology in drug target identification and validation.

Expert opinion on drug discovery·2017

Area of Science:

  • Drug discovery and development
  • Biotechnology
  • Assay development

Background:

  • High throughput screening (HTS) is fundamental to modern drug discovery.
  • Effective HTS requires integrating expertise from biology, chemistry, engineering, and informatics.
  • Numerous variables influence HTS success, from target selection to lead compound identification.

Purpose of the Study:

  • To emphasize the critical role of comprehensive HTS assay design and implementation.
  • To highlight the importance of strategic decision-making throughout the HTS process.
  • To underscore the need for balancing quality guidelines with pragmatic compromise in HTS.

Main Methods:

  • Interdisciplinary collaboration involving biologists, chemists, engineers, and informaticians.

More Related Videos

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
08:43

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

Published on: March 10, 2017

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
09:44

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

Published on: March 3, 2015

Related Experiment Videos

Last Updated: Jun 8, 2026

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography
06:19

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

Published on: March 10, 2023

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
08:43

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

Published on: March 10, 2017

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
09:44

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

Published on: March 3, 2015

  • Systematic analysis of variables influencing assay performance and outcome.
  • Development of quality guidelines and pragmatic approaches for decision-making.

    • Main Results:

    • HTS assay success is highly dependent on meticulous planning and execution.
    • Decisions made at each stage significantly impact the overall HTS outcome.
    • A balance between established guidelines and adaptive strategies is necessary for optimal results.

    Conclusions:

    • Comprehensive assay design and interdisciplinary teamwork are essential for successful HTS.
    • Pragmatic decision-making and compromise are vital for navigating the complexities of HTS.
    • Effective HTS strategies lead to the successful discovery of lead compounds in drug development.