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Updated: Jun 8, 2026

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Gut microbiota and related diseases: clinical features
Vincenzo Stanghellini1, Giovanni Barbara, Cesare Cremon
1Department of Clinical Medicine, St. Orsola-Malpighi Hospital, University of Bologna, Building No. 5, Via Massarenti, 9, 40138, Bologna, Italy. v.stanghellini@unibo.it
Abstract:
Intestinal microbiota is essential for gut homeostasis. Specifically, the microorganisms inhabiting the gut lumen interact with the intestinal immune system, supply key nutrients for the major components of the gut wall, and modulate energy metabolism. Host-microbiome interactions can be either beneficial or deleterious, driving gastrointestinal lymphoid tissue activities and shaping gut wall structures. This overview briefly focuses on the potential role played by abnormalities in gut microbiota and relative responses of the gastrointestinal tract in the determination of important pathological conditions such as the irritable bowel syndrome, inflammatory bowel diseases and colorectal cancer.
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