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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes
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A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

Published on: May 22, 2018

Protein microarrays for genome-wide posttranslational modification analysis.

Yifat Merbl1, Marc W Kirschner

  • 1Systems Biology Department, Harvard Medical School, Boston, MA, USA.

Wiley Interdisciplinary Reviews. Systems Biology and Medicine
|September 25, 2010
PubMed
Summary

Protein microarrays enable high-throughput analysis of protein interactions and modifications. This technology advances proteomic studies by overcoming limitations in studying protein concentrations and modifications in biological samples.

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Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins

Published on: January 8, 2018

Area of Science:

  • Biochemistry
  • Proteomics
  • Molecular Biology

Background:

  • Protein microarrays offer a high-throughput method for analyzing protein interactions, expression, and modifications.
  • Traditional methods face challenges with wide dynamic ranges of protein concentrations and property changes during modification.
  • Recent advances allow using functional mammalian cell extracts for post-translational modification (PTM) assays.

Purpose of the Study:

  • To review studies utilizing protein microarrays for post-translational modification (PTM) profiling.
  • To discuss the limitations and potential applications of protein microarray technology in proteomics.
  • To highlight the value of proteomic studies in understanding human physiology.

Main Methods:

  • Review of existing literature on protein microarray applications for PTM analysis.
  • Discussion of the technical aspects of immobilizing proteins and analyzing proteomes.
  • Exploration of assays using functional mammalian cell extracts for PTM profiling.

Main Results:

  • Protein microarrays effectively compare protein binding, expression, substrate specificities, and PTMs in parallel.
  • The technology overcomes limitations related to protein concentration dynamics and modification-induced property changes.
  • Functional cell extracts enable new approaches for large-scale biochemical analysis of protein modifications.

Conclusions:

  • Protein microarray technology is a powerful tool for comprehensive proteomic analysis.
  • Further development and application of protein microarrays can significantly advance our understanding of human physiology.
  • This technology provides valuable insights into the molecular mechanisms governing biological processes.