Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
Electrochemical Systems01:24

Electrochemical Systems

Electrochemical systems provide a fascinating insight into the dynamic interplay of charged species within various phases. One notable example is the interaction between a membrane permeable to K⁺ ions but not to Cl⁻ ions, separating an aqueous KCl solution from pure water. As K⁺ ions diffuse through the membrane, they generate net charges on each phase, leading to a potential difference between them.Similarly, when a piece of Zn is immersed in an aqueous ZnSO₄ solution, the Zn metal, composed...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A widely used CRP aptamer does not bind CRP, revealing a general route to surface-driven pseudoaffinity in biosensors.

Biosensors & bioelectronics·2026
Same author

Widespread omission of aptamer-target binding verification in aptasensor development: Consequences for sensor performance and the need for a K<sub>d</sub> gate.

Biosensors & bioelectronics·2026
Same author

Deterministic Error Propagation in Kinetic <i>K</i><sub>d</sub> Determination: General Theory with Application to Surface-Based Assays.

ACS sensors·2026
Same author

A Roadmap for Reliable Determination of Aptamer-Target Equilibrium Dissociation Constants (<i>K</i><sub>d</sub>).

ACS sensors·2026
Same author

Introducing Quantitative Assessment of Michaelis Constant (K<sub>m</sub>) Accuracy.

Chembiochem : a European journal of chemical biology·2025
Same author

5-Formylcytosine is not a prevalent RNA modification in mammalian cells.

Nature communications·2025

Related Experiment Video

Updated: Jun 8, 2026

Using Laser Scanning Microscopy to Determine Electromigration in Molybdenum Disilicide
09:41

Using Laser Scanning Microscopy to Determine Electromigration in Molybdenum Disilicide

Published on: May 23, 2025

Heat-associated field distortion in electro-migration techniques.

Christopher J Evenhuis, Michael U Musheev, Sergey N Krylov

    Analytical Chemistry
    |September 28, 2010
    PubMed
    Summary
    This summary is machine-generated.

    Heat-associated field distortion (HAFD) in electrophoresis was experimentally proven for the first time. This phenomenon, caused by uneven heat dissipation, significantly impacts electric field strength in electro-migration techniques, potentially causing errors.

    More Related Videos

    A Novel Method for In Situ Electromechanical Characterization of Nanoscale Specimens
    07:15

    A Novel Method for In Situ Electromechanical Characterization of Nanoscale Specimens

    Published on: June 2, 2017

    Related Experiment Videos

    Last Updated: Jun 8, 2026

    Using Laser Scanning Microscopy to Determine Electromigration in Molybdenum Disilicide
    09:41

    Using Laser Scanning Microscopy to Determine Electromigration in Molybdenum Disilicide

    Published on: May 23, 2025

    A Novel Method for In Situ Electromechanical Characterization of Nanoscale Specimens
    07:15

    A Novel Method for In Situ Electromechanical Characterization of Nanoscale Specimens

    Published on: June 2, 2017

    Area of Science:

    • Analytical Chemistry
    • Physical Chemistry

    Background:

    • Electrophoresis and other electro-migration techniques are vital in analytical sciences.
    • The electric field strength in these techniques is often assumed to be uniform.
    • Nonuniform heat dissipation may cause heat-associated field distortion (HAFD), but this has not been experimentally verified.

    Purpose of the Study:

    • To experimentally demonstrate and quantify heat-associated field distortion (HAFD) in electro-migration.
    • To investigate the impact of nonuniform heat dissipation on electric field strength in capillary electrophoresis (CE).

    Main Methods:

    • Utilized capillary electrophoresis (CE) with a specialized capillary featuring sections with varying heat dissipation efficiencies.
    • Measured and compared electric field strengths across different sections of the capillary under typical CE conditions.

    Main Results:

    • Successfully demonstrated HAFD for the first time through experimental evidence.
    • Observed an approximate 1.5-fold difference in electric field strength between capillary sections with different heat dissipation properties.
    • Confirmed HAFD as a significant phenomenon in typical CE electrolytes.

    Conclusions:

    • Heat-associated field distortion (HAFD) is a pronounced phenomenon in electro-migration techniques like CE.
    • HAFD can introduce errors and instabilities into electro-migration experiments.
    • Further research is needed to understand and mitigate HAFD for improved accuracy in analytical sciences.