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Related Concept Videos

Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

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Related Experiment Video

Updated: Jun 8, 2026

Investigation of Protein Recruitment to DNA Lesions Using 405 Nm Laser Micro-irradiation
12:29

Investigation of Protein Recruitment to DNA Lesions Using 405 Nm Laser Micro-irradiation

Published on: March 20, 2018

Microscopic implications of S-DNA.

Stephen Whitelam1, Phillip L Geissler, Sander Pronk

  • 1Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA. swhitelam@lbl.gov

Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics
|September 28, 2010
PubMed
Summary

Recent DNA overstretching experiments show strand separation at 65 pN. We argue that this is consistent with a dynamic coexistence of hybridized and unhybridized DNA forms, not ruling out S-DNA.

Area of Science:

  • Biophysics
  • Molecular Biology
  • Genetics

Background:

  • Recent experiments reveal DNA strand separation at 65 pN during overstretching.
  • This observation was interpreted as evidence against the existence of S-DNA.

Purpose of the Study:

  • To investigate the consistency of the S-DNA model with experimental observations of DNA overstretching.
  • To propose an alternative interpretation of DNA overstretching experiments.

Main Methods:

  • Theoretical analysis of DNA mechanics.
  • Modeling of DNA strand separation and hybridization.

Main Results:

  • The S-DNA model is consistent with DNA overstretching and strand unpeeling.
  • DNA overstretching does not require complete conversion to the S-form.

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Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)
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Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay
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Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay

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Last Updated: Jun 8, 2026

Investigation of Protein Recruitment to DNA Lesions Using 405 Nm Laser Micro-irradiation
12:29

Investigation of Protein Recruitment to DNA Lesions Using 405 Nm Laser Micro-irradiation

Published on: March 20, 2018

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)
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Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)

Published on: September 24, 2015

Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay
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Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay

Published on: February 3, 2022

  • A dynamic coexistence of hybridized and unhybridized DNA forms can explain experimental data.

    • Conclusions:

    • The S-DNA model remains a viable explanation for DNA overstretching phenomena.
    • Experimental observations can be rationalized by a dynamic coexistence of DNA forms.