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Related Experiment Video

Updated: Jun 8, 2026

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Published on: September 13, 2022

Nanometer axial resolution by three-dimensional supercritical angle fluorescence microscopy.

Christian M Winterflood¹, Thomas Ruckstuhl, Dorinel Verdes

¹Physikalisch-Chemisches Institut, Universität Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland.

Physical Review Letters

|September 28, 2010

Summary

We developed a noninvasive fluorescence microscopy technique achieving nanometer resolution along the optical axis. This method precisely measures fluorophore distance from a slide by analyzing emitted light angles.

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Last Updated: Jun 8, 2026

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Published on: September 13, 2022

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Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection

Published on: February 24, 2026

Area of Science:

Optics and Photonics
Biomedical Imaging
Microscopy

Background:

Accurate axial localization of fluorophores is crucial for 3D biological imaging.
Conventional microscopy techniques often struggle with achieving high resolution along the optical axis.

Purpose of the Study:

To introduce a novel noninvasive fluorescence microscopy method.
To demonstrate nanometer-scale resolution along the optical axis using this technique.

Main Methods:

Utilizing the influence of the microscope slide on fluorescence angular intensity distribution.
Measuring the proportion of light emitted below and above the critical angle of total internal reflection.

Main Results:

Achieved nanometer resolution along the optical axis.
Demonstrated the technique's capability for precise axial position determination.
Showcased the exponential dependence of above-critical angle light on fluorophore distance.

Conclusions:

The developed method offers a noninvasive approach for high-resolution axial localization.
This technique has potential applications in advanced biological and materials science imaging.