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Radical Autoxidation01:20

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Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
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L-Carnitine protects plasma components against oxidative alterations.

Joanna Kolodziejczyk1, Joanna Saluk-Juszczak, Barbara Wachowicz

  • 1Department of General Biochemistry, University of Lodz, Lodz, Poland.

Nutrition (Burbank, Los Angeles County, Calif.)
|September 28, 2010
PubMed
Summary

L-Carnitine demonstrates significant antioxidant properties by protecting plasma proteins and lipids from oxidative damage. This study reveals its potential to combat harmful effects caused by peroxynitrite in vitro.

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Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation
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Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation

Published on: January 28, 2021

Area of Science:

  • Biochemistry
  • Nutritional Science
  • Cardiovascular Research

Background:

  • L-Carnitine is a dietary supplement often used to enhance exercise performance.
  • Its precise biological mechanisms, particularly regarding cardiovascular health, remain incompletely understood.
  • Dietary supplements can influence various metabolic pathways.

Purpose of the Study:

  • To investigate the in vitro antioxidant properties of L-Carnitine.
  • To determine if L-Carnitine protects plasma proteins and lipids against oxidative and nitrative damage induced by peroxynitrite.
  • To quantify the effects on protein carbonyl groups, thiols, 3-nitrotyrosine, and lipid peroxidation.

Main Methods:

  • Colorimetric assay for carbonyl group generation.
  • Enzyme-linked immunosorbent assay for 3-nitrotyrosine formation.
  • Spectrophotometry for lipid peroxidation (thiobarbituric-acid reactive substances).
  • High-performance liquid chromatography for thiol group analysis (plasma proteins, glutathione, cysteine, homocysteine).

Main Results:

  • L-Carnitine inhibited peroxynitrite-induced oxidation and nitration of plasma proteins in vitro.
  • It protected against the decrease in protein thiol (-SH) levels caused by peroxynitrite.
  • L-Carnitine also preserved low-molecular-weight thiols and protected plasma lipids from peroxidation.

Conclusions:

  • L-Carnitine exhibits significant in vitro antioxidant activity.
  • It effectively protects plasma components from oxidative and nitrative stress induced by peroxynitrite.