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Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
Published on: October 15, 2013
High-redundancy draft sequencing of 15 clinical and environmental Burkholderia strains
Sanghamitra Mukhopadhyay1, Maureen K Thomason, Shannon Lentz
1Biological Defense Research Directorate, Naval Medical Research Center, Silver Spring, Maryland 20910, USA.
Journal of Bacteriology
|September 28, 2010
Abstract:
The Gram-negative Burkholderia genus includes several species of intracellular bacterial pathogens that pose substantial risk to humans. In this study, we have generated draft genome sequences of 15 strains of B. oklahomensis, B. pseudomallei, B. thailandensis, and B. ubonensis to an average sequence read coverage of 25- to 40-fold.
