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The carboxysome shell is permeable to protons.

Balaraj B Menon1, Sabine Heinhorst, Jessup M Shively

  • 1Department of Chemistry and Biochemistry, The University of Southern Mississippi, Hattiesburg, MS 39406, USA.

Journal of Bacteriology
|September 28, 2010
PubMed
Summary
This summary is machine-generated.

Bacterial microcompartments (BMCs) do not maintain a lower internal pH. Researchers found that the protein shell of BMCs is permeable to protons, suggesting their function is not pH optimization.

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Published on: March 30, 2015

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Background:

  • Bacterial microcompartments (BMCs) are protein-bound organelles found in bacteria.
  • They compartmentalize metabolic pathways, such as those in carboxysomes.
  • Unlike eukaryotic organelles, BMCs have protein shells, not lipid bilayers.

Purpose of the Study:

  • To investigate the proposed function of BMCs in maintaining a lower internal pH.
  • To determine if the protein shell of BMCs acts as a proton barrier.

Main Methods:

  • Recombinant fusion of pH-sensitive green fluorescent protein (GFP) to ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO).
  • Measurement of internal carboxysomal pH relative to the cytoplasm.

Main Results:

  • Carboxysomal pH was found to be similar to the external environment.
  • The protein shell of BMCs does not significantly impede proton flux.

Conclusions:

  • The hypothesis that BMCs maintain a lower internal pH for metabolic advantage is not supported.
  • The function of BMCs likely relies on other properties, such as the characteristics of their shell pores.