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Visualizing Bacteria in Nematodes using Fluorescent Microscopy
Published on: October 19, 2012
'Candidatus Pasteuria aldrichii', an obligate endoparasite of the bacterivorous nematode Bursilla
R M Giblin-Davis1, G Nong2, J F Preston2
1University of Florida-IFAS, Fort Lauderdale Research and Education Center, 3205 College Avenue, Fort Lauderdale, FL 33314-7719, USA.
Abstract:
A novel bacterium of the genus Pasteuria was discovered parasitizing bacterivorous nematodes of the genus Bursilla, in selected bermudagrass (Cynodon) field plots in Davie, FL, USA. Soil containing this bacterium was sampled and supplied with bi-weekly inoculations of cultured species of the genus Bursilla in order to build and maintain a source of endospores for continuous in vivo conservation of the bacteria for further study and characterization. 16S rRNA gene sequence similarities supported its congeneric ranking with other members of the genus Pasteuria that have been identified from nematodes and cladocerans. There were, however, no clear sister candidates for this organism, which supported the evidence of endospore ultrastructure and host-range studies, suggesting it belonged to a novel taxon. Because members of the genus Pasteuria cannot yet be isolated, definitive type strains could not be maintained; therefore, the name 'Candidatus Pasteuria aldrichii' is proposed for this organism.
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