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Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

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Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
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Published on: May 25, 2015

MicroRNAs: tiny targets for engineering CHO cell phenotypes?

Niall Barron1, Noelia Sanchez, Paul Kelly

  • 1National Institute for Cellular Biotechnology, Dublin City University, Dublin 9, Ireland. niall.barron@dcu.ie

Biotechnology Letters
|September 28, 2010
PubMed
Summary
This summary is machine-generated.

MicroRNAs regulate gene expression and impact biological processes, showing promise as biomarkers and therapeutic targets. This review covers microRNA study methods and applications in bioprocessing, especially for Chinese hamster ovary cell engineering.

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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biotechnology

Background:

  • MicroRNAs (miRNAs) are key regulators of gene expression, influencing most cellular processes.
  • Their significant biological impact has led to interest in miRNAs as biomarkers and therapeutic targets.

Purpose of the Study:

  • To provide an overview of miRNA biogenesis, function, and expression analysis.
  • To explore the application of miRNAs as tools in bioprocessing, with a focus on Chinese hamster ovary (CHO) cell engineering.

Main Methods:

  • Review of literature on miRNA biogenesis, genomic organization, and mechanisms of action.
  • Description of methods for studying miRNA expression.
  • Discussion of strategies for utilizing miRNAs in biotechnology and bioprocessing.

Main Results:

  • MicroRNAs play a fundamental role in cellular gene regulation.
  • Various methods exist for studying miRNA expression profiles.
  • Potential applications in bioprocessing, particularly for enhancing CHO cell productivity, are being explored.

Conclusions:

  • MicroRNAs are crucial for gene regulation and have broad biological implications.
  • Understanding miRNA expression and function is vital for their application.
  • Further research into miRNA engineering holds promise for advancing bioprocessing technologies, especially in CHO cell lines.