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Characterizing Multidrug Efflux Systems in Acinetobacter baumannii Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System
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Characterizing Multidrug Efflux Systems in Acinetobacter baumannii Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

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[Carbapenem-resistant Acinetobacter baumannii strains]

Tomasz Bogiel1, Joanna Kwiecińska-Piróg, Katarzyna Jachna-Sawicka

  • 1Katedra i Zakład Mikrobiologii Collegium Medicum im. L. Rydygiera w Bydgoszczy Uniwersytet Mikolaja Kopernika w Toruniu.

Medycyna Doswiadczalna I Mikrobiologia
|September 30, 2010
PubMed

Insights

Carbapenem-resistant Acinetobacter baumannii (CRAB) infections are increasing, particularly in hospital settings. Colistin and tobramycin showed the most promise against these resistant strains.

Area of Science:

  • Medical Microbiology
  • Infectious Diseases
  • Antimicrobial Resistance

Background:

  • Acinetobacter baumannii is an opportunistic pathogen frequently causing nosocomial infections.
  • Carbapenem resistance in A. baumannii poses a significant threat due to inter-species transmissibility.

Purpose of the Study:

  • To evaluate the isolation frequency and antibiotic susceptibility of imipenem- and/or meropenem-resistant A. baumannii strains.
  • To analyze trends in CRAB infections between 2007 and 2009 at a university hospital.

Main Methods:

  • Retrospective analysis of A. baumannii isolates from 2007-2009.
  • Antimicrobial susceptibility testing using standard microbiological methods.
  • Data collection on patient demographics and sample sources.

Main Results:

  • A significant increase in CRAB isolation frequency was observed, from 4 strains in 2007 to 95 in 2008 and 67 in 2009.
  • Imipenem resistance rose to 27.6% in 2008 and 31.0% in 2009; meropenem resistance reached 31.2% and 34.6% in 2008 and 2009, respectively.
  • Highest in vitro activity against CRAB was demonstrated by colistin (1.5% resistance), tobramycin (8.1% resistance), and ampicillin/sulbactam (24.8% resistance).

Conclusions:

  • CRAB infections show a concerning increasing trend, predominantly in Intensive Care Units and surgical clinics.
  • CRAB strains exhibit high resistance to common antibiotics like fluoroquinolones and chloramphenicol.
  • Colistin and tobramycin remain the most effective therapeutic options against CRAB isolates in this study.

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