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Published on: November 1, 2017
Immunoliposomal systems targeting primary effusion lymphoma: in vitro study
Barbara Ruozi1, Giovanni Riva, Daniela Belletti
1Department of Pharmaceutical Sciences, University of Modena & Reggio Emilia, Via Campi 183, Modena, Italy. barbara.ruozi@unimo.it
Aims:
To develop an appropriate liposomal formulation for gene delivery against primary effusion lymphoma (PEL), a herpesvirus HHV8-associated B-cell lymphoma.
Materials & Methods:
Cationic, cationic PEGylated and cationic PEGylated anti-CD138 liposomes (ILp) linking a monoclonal antibody expressed on PEL cells were prepared by a thin layer evaporation method, followed by extrusion technique. The formulations were mixed with a model oligonucleotide to form the lipoplexes and tested on a BCBL-1 cell (a PEL cell line). The transfection efficiency was evaluated by flow cytometry and confocal laser scanning microscopy analysis.
Results:
Based on antigen-antibody interaction, ILp mediated a specific gene delivery as shown by a significant increase in the transfection rate and a localized internalization of the oligonucleotide, in comparison with cationic liposomes and cationic PEGylated liposomes.
Conclusion:
ILp could be proposed as effective carriers for oligonucleotide transfer in BCBL-1 cells. In vitro experimental results encourage us to further test the in vivo therapeutic potential of ILp for specific delivery of antitumoral agents.
