Related Experiment Video
Updated: Feb 23, 2026
09:09
Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
Published on: May 2, 2019
8.0K
Smart siRNA delivery systems based on polymeric nanoassemblies and nanoparticles.
Atsushi Tamura1, Yukio Nagasaki
1Graduate School of Pure & Applied Sciences, University of Tsukuba. 1-1-1 Ten-noudai, Tsukuba, Ibaraki, Japan.
Nanomedicine (London, England)
|September 30, 2010
Summary
Small interfering RNA (siRNA) holds therapeutic promise for intractable diseases. This review explores advanced polyion complex delivery systems designed to overcome siRNA
Area of Science:
- Biochemistry
- Molecular Biology
- Nanotechnology
Background:
- RNA interference (RNAi) utilizes small interfering RNA (siRNA) for post-transcriptional gene silencing.
- siRNA therapeutics offer novel treatment strategies for intractable diseases.
- Systemic application of siRNA is limited by enzymatic instability, cellular uptake, and biodistribution challenges.
Purpose of the Study:
- To review recent advancements in siRNA delivery systems.
- To highlight strategies for overcoming limitations in systemic siRNA therapy.
- To focus on polyion complex-based siRNA delivery systems.
Main Methods:
- Development of siRNA-loaded polyion complexes via electrostatic interactions.
- Incorporation of synthetic polymers, biopolymers, and nanoparticles as positively charged components.
- Design of stimulus-sensitive carriers, including bioreducible polycations and polymer-siRNA conjugates.
Main Results:
- Numerous polyion complex systems have been engineered for enhanced siRNA delivery.
- Stimulus-sensitive systems demonstrate improved gene-silencing efficacy.
- Polyion complexes address key challenges in enzymatic tolerability and biodistribution.
Conclusions:
- Polyion complexes represent a promising strategy for effective siRNA therapeutics.
- Advanced carrier systems are crucial for overcoming systemic delivery barriers.
- Further development of smart siRNA delivery systems will enhance therapeutic applications.
More Related Videos
Related Concept Videos
01:24Site-Targeted Drug Delivery Systems: Polymeric Carriers
37
Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...
37
01:24Modified-Release Drug Delivery Systems: Site-Targeted
33
Site-targeted drug delivery systems enhance therapeutic efficacy while minimizing systemic toxicity and treatment costs. Unlike conventional methods, these systems ensure precise drug delivery, improving bioavailability and reducing side effects. Targeted drug delivery is classified into three levels. First-order targeting directs drugs to the capillary beds of specific organs or tissues. Second-order targets specific cell types, such as tumor cells, using receptor-mediated interactions.
33
02:30siRNA - Small Interfering RNAs
18.8K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
18.8K