Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase II Reactions: Glutathione Conjugation and Mercapturic Acid Formation01:22

Phase II Reactions: Glutathione Conjugation and Mercapturic Acid Formation

Glutathione, a tripeptide made up of glutamate, cysteine, and glycine, is a critical player in the detoxification of drugs and xenobiotics via a process known as glutathione conjugation or mercapturic acid formation. This phase II biotransformation reaction involves the covalent binding of glutathione to a drug or its metabolite, enhancing the compound's water solubility and enabling its excretion.
Several distinctive characteristics distinguish glutathione conjugation from other phase II...
The Cell Cycle Control System01:28

The Cell Cycle Control System

The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
Sulfur Assimilation01:20

Sulfur Assimilation

Sulfur is an essential element in biological systems, contributing to synthesizing key biomolecules, including amino acids such as cysteine and methionine, and cofactors such as coenzyme A and biotin. Microorganisms primarily assimilate sulfur as sulfate (SO₄²⁻) from the environment, which must undergo a series of biochemical transformations before it can be incorporated into cellular components. As sulfate is highly oxidized, it must undergo assimilatory sulfate reduction to become...
What is the Cell Cycle?00:56

What is the Cell Cycle?

The cell cycle refers to the sequence of events occurring throughout a typical cell’s life. In eukaryotic cells, the somatic cell cycle has two stages: the interphase and the mitotic phase. During interphase, the cell grows, performs its basic metabolic functions, copies its DNA, and prepares for mitotic cell division. Then, during mitosis and cytokinesis, the cell divides its nuclear and cytoplasmic materials, respectively. This generates two daughter cells that are identical to the original...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chloroplast photorespiratory bypass in tomato couples carbon-nitrogen assimilation to increase yield and fruit quality.

Cell reports·2026
Same author

Plant development: RBOH genes.

Current biology : CB·2026
Same author

Validation of circulating miR-323a-3p and miR-625-3p to classify hypertrophic cardiomyopathy in Friedreich's ataxia.

Scientific reports·2026
Same author

From the lab to lifestyle: epigenetic clocks in personalized aging and health.

Biogerontology·2026
Same author

Reactive oxygen species and oxidative signalling in plants.

Essays in biochemistry·2026
Same author

Emerging concepts of reactive oxygen species functions in plants.

Biochemical Society transactions·2026

Related Experiment Video

Updated: Jun 8, 2026

Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro
03:35

Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro

Published on: June 28, 2024

A nuclear glutathione cycle within the cell cycle.

Pedro Diaz Vivancos1, Tonja Wolff, Jelena Markovic

  • 1CEBAS-CSIC, Department of Plant Breeding, Murcia, Campus de Espinardo, Spain.

The Biochemical Journal
|September 30, 2010
PubMed
Summary

Glutathione (GSH) in the nucleus regulates cell proliferation and gene expression by maintaining cellular redox balance. Nuclear GSH influences genetic and epigenetic events, potentially creating a transgenerational memory of the cellular redox environment.

More Related Videos

The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins
09:22

The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins

Published on: December 13, 2013

Spectrophotometric Screening for Potential Inhibitors of Cytosolic Glutathione S-Transferases
14:57

Spectrophotometric Screening for Potential Inhibitors of Cytosolic Glutathione S-Transferases

Published on: October 10, 2020

Related Experiment Videos

Last Updated: Jun 8, 2026

Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro
03:35

Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro

Published on: June 28, 2024

The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins
09:22

The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins

Published on: December 13, 2013

Spectrophotometric Screening for Potential Inhibitors of Cytosolic Glutathione S-Transferases
14:57

Spectrophotometric Screening for Potential Inhibitors of Cytosolic Glutathione S-Transferases

Published on: October 10, 2020

Area of Science:

  • Cell Biology
  • Biochemistry
  • Molecular Biology

Background:

  • The thiol tripeptide glutathione (GSH) is central to cellular antioxidant networks, buffering reactive oxygen species (ROS) and mediating redox signaling.
  • GSH is present in all cellular compartments, including the nucleus, where its transport is crucial for regulating cell proliferation.

Purpose of the Study:

  • To review the functions of nuclear GSH (GSHn) in cellular redox homeostasis and signaling.
  • To explore potential mechanisms for GSH transport into the nucleus.
  • To discuss the role of GSHn in regulating nuclear proteins and epigenetic events.

Main Methods:

  • Literature review focusing on cellular redox signaling, cell cycle regulation, and nuclear transport.
  • Analysis of existing research on GSH localization and function within the nucleus.
  • Discussion of potential regulatory roles of GSHn on histones and PARP.

Main Results:

  • GSH co-localizes with nuclear DNA during early proliferation stages in plant and animal cells.
  • Nuclear sequestration of GSH during G1 and S phases impacts cell proliferation and gene expression, decreasing stress/defense protein transcripts.
  • GSHn may regulate nuclear proteins like histones and PARP, influencing genetic and epigenetic processes.

Conclusions:

  • Nuclear GSH plays a critical role in maintaining cellular redox homeostasis and regulating gene expression.
  • GSHn influences cell proliferation and may contribute to a heritable cellular redox memory through epigenetic mechanisms.
  • Understanding GSH nuclear transport and function is key to comprehending cellular responses to redox environments.