Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Replication in Prokaryotes02:35

Replication in Prokaryotes

Overview
Replication in Prokaryotes01:32

Replication in Prokaryotes

DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
DNA Replication02:40

DNA Replication

DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication uses a large number of...
Reproductive Cloning01:27

Reproductive Cloning

Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

First Draft Genome Sequence of the Pathogenic Fungus <i>Lomentospora prolificans</i> (Formerly <i>Scedosporium prolificans</i>).

G3 (Bethesda, Md.)·2017
Same author

Support Science by Publishing in Scientific Society Journals.

mBio·2017
Same author

Lipids Affect the Cryptococcus neoformans-Macrophage Interaction and Promote Nonlytic Exocytosis.

Infection and immunity·2017
Same author

Forensic bitemark identification: weak foundations, exaggerated claims.

Journal of law and the biosciences·2017
Same author

Correction: Macrophage Mitochondrial and Stress Response to Ingestion of Cryptococcus neoformans.

Journal of immunology (Baltimore, Md. : 1950)·2017
Same author

Microbial melanins for radioprotection and bioremediation.

Microbial biotechnology·2017
Same journal

<i>Porphyromonas gingivalis</i> sphingolipids affect early responses of THP-1 macrophages to outer membrane vesicles.

Infection and immunity·2026
Same journal

A genome-wide CRISPR screen defines host determinants of early <i>Brucella</i> infection in human macrophage-like cells.

Infection and immunity·2026
Same journal

Antiphospholipid antibodies in acute and post-treatment Lyme disease.

Infection and immunity·2026
Same journal

The cholesterol-dependent cytolysin promotes <i>Streptococcus</i> systemic spread and induces arachidonic acid accumulation-mediated lethality in a murine intraperitoneal infection model.

Infection and immunity·2026
Same journal

Phenotypic and genotypic analysis of <i>Candida albicans</i> vaginal isolates reveals that <i>ECE1</i> expression underpins pathogenicity.

Infection and immunity·2026
Same journal

<i>Staphylococcus aureus</i> pore-forming toxins differentially shape disease severity in experimental endophthalmitis.

Infection and immunity·2026
See all related articles

Related Experiment Video

Updated: Jun 8, 2026

An Open Source Technology Platform to Manufacture Hydrogel-Based 3D Culture Models in an Automated and Standardized Fashion
08:29

An Open Source Technology Platform to Manufacture Hydrogel-Based 3D Culture Models in an Automated and Standardized Fashion

Published on: March 31, 2022

Reproducible science.

Arturo Casadevall, Ferric C Fang

    Infection and Immunity
    |September 30, 2010
    PubMed
    Summary
    This summary is machine-generated.

    Scientific reproducibility is crucial but challenging due to complex biological systems. This essay examines the importance and limitations of ensuring reproducible experimental results in scientific publications.

    More Related Videos

    Reproducibility and Harmonization in Research Using Biological Standards: The Example of Platelet Agonist Collagen-Related Peptide
    04:50

    Reproducibility and Harmonization in Research Using Biological Standards: The Example of Platelet Agonist Collagen-Related Peptide

    Published on: August 4, 2023

    Related Experiment Videos

    Last Updated: Jun 8, 2026

    An Open Source Technology Platform to Manufacture Hydrogel-Based 3D Culture Models in an Automated and Standardized Fashion
    08:29

    An Open Source Technology Platform to Manufacture Hydrogel-Based 3D Culture Models in an Automated and Standardized Fashion

    Published on: March 31, 2022

    Reproducibility and Harmonization in Research Using Biological Standards: The Example of Platelet Agonist Collagen-Related Peptide
    04:50

    Reproducibility and Harmonization in Research Using Biological Standards: The Example of Platelet Agonist Collagen-Related Peptide

    Published on: August 4, 2023

    Area of Science:

    • * Integrative Biology
    • * Scientific Methodology

    Background:

    • * Reproducibility is a cornerstone of scientific validity and trust.
    • * Confirmatory findings often face publication bias, hindering scientific progress.
    • * The inherent complexity of biological systems presents significant challenges to experimental replication.

    Discussion:

    • * This essay critically evaluates the essential role of reproducibility in scientific manuscripts.
    • * It delves into the inherent limitations and practical difficulties in achieving perfect replicability in biological research.
    • * The discussion highlights the tension between the ideal of reproducibility and the realities of scientific publication and biological complexity.

    Key Insights:

    • * Reproducibility is fundamental, yet often undervalued in scientific publishing.
    • * Biological system complexity inherently limits the scope of experimental replication.
    • * Balancing the pursuit of novelty with the need for reproducibility is essential for scientific integrity.

    Outlook:

    • * Future research must address strategies to enhance reproducibility in complex biological studies.
    • * Developing standardized reporting guidelines can improve the clarity and replicability of experimental methods.
    • * Fostering a scientific culture that values and incentivizes reproducibility is paramount for long-term scientific advancement.