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Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Microtubules in Signaling01:22

Microtubules in Signaling

The primary cilium, made up of microtubules, acts as antennae on the cell surfaces for relaying external stimuli into the cells. These fine hair-like structures are present, generally one per cell. These are non-motile cilia in a 9+0 microtubules arrangement, where the central pair of microtubules are absent. The primary cilia arise from the basal body embedded in the cell membrane. Intraflagellar transport (IFT) carries requisite proteins from the cytoplasm to the cilium because the primary...
Mechanism of Ciliary Motion01:05

Mechanism of Ciliary Motion

The ciliary structures were first seen in 1647 by Antonie Leeuwenhoek while observing the protozoans. In lower organisms, these appendages are responsible for cell movement, while in higher organisms, these appendages help in the movement of the extracellular fluids within the body cavities.
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
Mechanism of Ciliary Motion01:05

Mechanism of Ciliary Motion

The ciliary structures were first seen in 1647 by Antonie Leeuwenhoek while observing the protozoans. In lower organisms, these appendages are responsible for cell movement, while in higher organisms, these appendages help in the movement of the extracellular fluids within the body cavities.
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...

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Related Experiment Video

Updated: Jun 8, 2026

Antibody Uptake Assay for Tracking Notch/Delta Endocytosis During the Asymmetric Division of Zebrafish Radial Glia Progenitors
09:06

Antibody Uptake Assay for Tracking Notch/Delta Endocytosis During the Asymmetric Division of Zebrafish Radial Glia Progenitors

Published on: January 20, 2023

Notch signalling regulates left-right asymmetry through ciliary length control.

Susana S Lopes1, Raquel Lourenço, Luís Pacheco

  • 1Instituto de Medicina Molecular e Instituto de Histologia e Biologia do Desenvolvimento, Faculdade de Medicina da Universidade de Lisboa, 1649-028 Lisboa, Portugal.

Development (Cambridge, England)
|September 30, 2010
PubMed
Summary

Notch signaling controls cilia length, crucial for embryonic development and organ laterality. This study reveals how Notch impacts cilia, fluid dynamics, and gene expression, offering insights into human ciliopathies.

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Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer's Vesicle
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Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer's Vesicle

Published on: March 31, 2013

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Last Updated: Jun 8, 2026

Antibody Uptake Assay for Tracking Notch/Delta Endocytosis During the Asymmetric Division of Zebrafish Radial Glia Progenitors
09:06

Antibody Uptake Assay for Tracking Notch/Delta Endocytosis During the Asymmetric Division of Zebrafish Radial Glia Progenitors

Published on: January 20, 2023

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis
07:26

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis

Published on: January 31, 2025

Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer's Vesicle
08:11

Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer's Vesicle

Published on: March 31, 2013

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Genetics

Background:

  • Human ciliopathies highlight the critical roles of cilia in development and physiology.
  • Molecular mechanisms regulating cilia formation remain incompletely understood.

Purpose of the Study:

  • To investigate the role of Notch signaling in controlling cilia length.
  • To elucidate the relationship between cilia length, fluid dynamics, and gene expression in embryonic development.

Main Methods:

  • Utilized deltaD zebrafish mutants to study cilia length and function.
  • Assessed the impact of Notch signaling modulation on cilia.
  • Analyzed fluid flow velocity and gene expression in Kupffer's vesicle.

Main Results:

  • Notch signaling is identified as a key regulator of cilia length.
  • Reduced cilia length in deltaD mutants impairs fluid flow and asymmetric gene expression.
  • Hyperactivation of Notch signaling leads to increased cilia length.
  • Disclosed an uncoupling between gut and heart laterality in deltaD mutants.

Conclusions:

  • Notch signaling integrates cilia length control with fluid dynamics to regulate laterality gene expression.
  • Findings provide insights into the molecular basis of ciliopathies and organ development.