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Updated: Jun 8, 2026
Key Elements of Photo Attraction Bioassay for Insect Studies or Monitoring Programs
Published on: July 26, 2018
Atomic force microscopy study of nano-physiological response of ladybird beetles to photostimuli
Natalia V Guz1, Maxim E Dokukin, Igor Sokolov
1Department of Physics, Clarkson University, Potsdam, New York, USA.
Background:
Insects are of interest not only as the most numerous and diverse group of animals but also as highly efficient bio-machines varying greatly in size. They are the main human competitors for crop, can transmit various diseases, etc. However, little study of insects with modern nanotechnology tools has been done.
Methodology/Principal Findings:
Here we applied an atomic force microscopy (AFM) method to study stimulation of ladybird beetles with light. This method allows for measuring of the internal physiological responses of insects by recording surface oscillations in different parts of the insect at sub-nanometer amplitude level and sub-millisecond time. Specifically, we studied the sensitivity of ladybird beetles to light of different wavelengths. We demonstrated previously unknown blindness of ladybird beetles to emerald color (∼500nm) light, while being able to see UV-blue and green light. Furthermore, we showed how one could study the speed of the beetle adaptation to repetitive flashing light and its relaxation back to the initial stage.
Conclusions:
The results show the potential of the method in studying insects. We see this research as a part of what might be a new emerging area of "nanophysiology" of insects.
