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Cholesterol: Significance and Regulation01:29

Cholesterol: Significance and Regulation

Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
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Updated: Jun 8, 2026

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol
10:12

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol

Published on: March 25, 2020

Cholesterol pathways affected by small molecules that decrease sterol levels in Niemann-Pick type C mutant cells.

Madalina Rujoi1, Nina H Pipalia, Frederick R Maxfield

  • 1Department of Biochemistry, Weill Cornell Medical College, New York, New York, USA.

Plos One
|September 30, 2010
PubMed
Summary
This summary is machine-generated.

Researchers identified cholesterol-lowering compounds for Niemann-Pick type C (NPC) disease. These compounds reduce cholesterol in NPC cells by enhancing efflux, decreasing uptake, or increasing esterification, aiding future therapeutic target identification.

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Last Updated: Jun 8, 2026

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol
10:12

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Published on: March 25, 2020

LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring
08:45

LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring

Published on: November 17, 2018

Cholesterol Efflux Assay
07:54

Cholesterol Efflux Assay

Published on: March 6, 2012

Area of Science:

  • Biochemistry
  • Cell Biology
  • Genetics

Background:

  • Niemann-Pick type C (NPC) disease is a genetic disorder characterized by impaired cholesterol efflux.
  • This leads to multi-lipid storage in lysosomal organelles.

Purpose of the Study:

  • To investigate cholesterol-lowering compounds for NPC disease.
  • To understand their effects on sterol pathways in NPC1-deficient cells.

Main Methods:

  • Utilized CT60 mutant Chinese hamster ovary (CHO) cells lacking NPC1 protein.
  • Screened selected cholesterol-lowering compounds for their effects on cellular cholesterol levels and pathways.

Main Results:

  • Identified compounds that decrease lysosomal cholesterol in NPC1 mutant cells.
  • Mechanisms include increased cholesterol efflux, decreased low-density lipoprotein (LDL) uptake, and increased cholesteryl ester levels.
  • Several compounds promoted cholesterol efflux to extracellular acceptors in both normal and NPC1 mutant cells.

Conclusions:

  • The study identified seven novel inhibitors of lysosomal acid lipase.
  • Provides a basis for prioritizing further research into molecular targets for NPC disease therapies.