Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Assembly of the cell nucleus.

R A Laskey1, G H Leno

  • 1Department of Zoology, University of Cambridge, UK.

Trends in Genetics : TIG
|December 1, 1990
PubMed
Summary

Researchers can assemble purified DNA into structures mimicking cell nuclei using cell-free systems. These systems enable the study of nuclear component assembly and the analysis of nuclear function.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Obesity-related endometrial cancer: an update on survivorship approaches to reducing cardiovascular death.

BJOG : an international journal of obstetrics and gynaecology·2016
Same author

Diagnosis of bladder cancer by immunocytochemical detection of minichromosome maintenance protein-2 in cells retrieved from urine.

British journal of cancer·2012
Same author

Chromatin configurations in the ferret germinal vesicle that reflect developmental competence for in vitro maturation.

Reproduction in domestic animals = Zuchthygiene·2008
Same author

MCM immunocytochemistry as a first line cervical screening test in developing countries: a prospective cohort study in a regional cancer centre in India.

British journal of cancer·2007
Same author

A minimally invasive immunocytochemical approach to early detection of oral squamous cell carcinoma and dysplasia.

British journal of cancer·2006
Same author

Aberrant expression of minichromosome maintenance protein-2 and Ki67 in laryngeal squamous epithelial lesions.

British journal of cancer·2003

Area of Science:

  • Cell biology
  • Molecular biology
  • Biochemistry

Background:

  • Cell nuclei are essential organelles housing genetic material.
  • Understanding nuclear assembly and function is crucial in cell biology.
  • Existing methods for studying nuclear components have limitations.

Purpose of the Study:

  • To investigate the assembly of cell nuclei from purified DNA.
  • To explore the utility of cell-free systems for nuclear research.
  • To analyze nuclear function through experimental manipulation.

Main Methods:

  • Utilizing cell-free systems for DNA self-assembly.
  • Employing techniques to purify DNA components.
  • Developing experimental setups to probe nuclear function in vitro.

Main Results:

  • Successfully assembled DNA structures closely resembling native cell nuclei.
  • Demonstrated the feasibility of studying nuclear component assembly pathways.
  • Showcased the potential for experimental analysis of nuclear functions in these systems.

Conclusions:

  • Cell-free systems provide a powerful platform for reconstructing and studying cell nuclei.
  • This approach facilitates research into the fundamental processes of nuclear assembly and function.
  • Offers new avenues for dissecting the complexities of nuclear biology.

Related Experiment Videos