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Related Experiment Videos

Chemotaxis in Dictyostelium discoideum: effect of concanavalin A on chemoattractant mediated cyclic gmp accumulation

J M Mato, P J van Haastert, F A Krens

    Cell Biology International Reports
    |March 1, 1978
    PubMed
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    Concanavalin A (Con A) affects cellular slime mold Dictyostelium discoideum responses to folic acid and cyclic AMP. This study proposes a model for chemotaxis based on these Con A-induced alterations in cell signaling.

    Area of Science:

    • Cell biology
    • Biochemistry
    • Microbiology

    Background:

    • Cellular slime molds like Dictyostelium discoideum are model organisms for studying cell signaling and chemotaxis.
    • Chemotaxis, the directed movement of cells in response to chemical stimuli, is crucial for development and immune responses.
    • Folic acid and cyclic AMP are key chemoattractants in Dictyostelium discoideum.

    Purpose of the Study:

    • To investigate the effects of concanavalin A (Con A) on Dictyostelium discoideum cell responses to chemoattractants.
    • To elucidate the role of Con A in modulating cyclic AMP and folic acid-mediated signaling pathways.
    • To propose a model for chemotaxis based on observed cellular responses.

    Main Methods:

    • Treatment of Dictyostelium discoideum cells with varying concentrations of Con A (100 µg/ml and 10 µg/ml).

    Related Experiment Videos

  • Measurement of light scattering changes induced by folic acid and cyclic AMP.
  • Quantification of cyclic GMP accumulation mediated by folic acid and cyclic AMP.
  • Analysis of cellular responses to repeated cyclic AMP stimulation.

    • Main Results:

    • Concanavalin A (100 µg/ml) inhibited folic acid and cyclic AMP-induced decreases in light scattering.
    • Con A (100 µg/ml) did not affect folic acid-mediated cyclic GMP accumulation but doubled cyclic AMP-mediated cyclic GMP accumulation.
    • At a lower concentration (10 µg/ml), Con A altered cyclic AMP responses, leading to a monophasic instead of biphasic light scattering change, while still stimulating cyclic GMP accumulation.
    • Repeated cyclic AMP stimulation resulted in light scattering decrease without concurrent changes in cyclic GMP concentration.

    Conclusions:

    • Concanavalin A significantly modulates chemoattractant-induced signaling pathways in Dictyostelium discoideum.
    • The observed effects of Con A suggest its involvement in regulating cellular responses critical for chemotaxis.
    • The findings provide a basis for a proposed model of chemotaxis in cellular slime molds.