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Related Experiment Video

Updated: Jun 8, 2026

Imaging Intracellular Ca2+ Signals in Striatal Astrocytes from Adult Mice Using Genetically-encoded Calcium Indicators
12:19

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Published on: November 19, 2014

Growth factors upregulate astrocyte [Ca2+]i oscillation by increasing SERCA2b expression.

Mitsuhiro Morita1, Yoshihisa Kudo

  • 1Department of Neurosurgery, University of New Mexico, Albuquerque, New Mexico, USA. mmorita@boar.kobe-u.ac.jp.

Glia
|September 30, 2010
PubMed
Summary

Growth factors enhance astrocyte calcium oscillations by upregulating sarco-endoplasmic reticulum Ca(2+) ATPase (SERCA) activity, specifically SERCA2b expression. This mechanism increases intracellular calcium storage, modulating calcium signaling patterns.

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Area of Science:

  • Neuroscience
  • Cellular Biology
  • Biochemistry

Background:

  • Growth factors like EGF and bFGF were previously shown to induce calcium ([Ca(2+)](i)) oscillations in astrocytes.
  • These growth factors also increase intracellular calcium stores, suggesting a link to calcium signaling modulation.

Purpose of the Study:

  • To investigate the mechanism by which growth factors upregulate astrocyte calcium ([Ca(2+)](i)) oscillations.
  • To identify the role of sarco-endoplasmic reticulum Ca(2+) ATPase (SERCA) in mediating growth factor-induced calcium oscillations.

Main Methods:

  • Assessed the effect of growth factors on SERCA activity using a SERCA inhibitor.
  • Measured intracellular calcium ([Ca(2+)](i)) levels and oscillations in response to glutamate.
  • Quantified the expression of SERCA subtypes, focusing on SERCA2b.

Main Results:

  • Growth factors upregulated a SERCA inhibitor-sensitive component of calcium ([Ca(2+)](i)) clearance.
  • Expression of the SERCA subtype, SERCA2b, was increased by growth factor treatment.
  • Partial inhibition of SERCA activity reversed growth factor-induced calcium oscillations back to sustained increases.

Conclusions:

  • Upregulation of sarco-endoplasmic reticulum Ca(2+) ATPase (SERCA) activity, particularly SERCA2b, is responsible for the enhanced calcium ([Ca(2+)](i)) oscillations in growth factor-treated astrocytes.
  • Growth factors regulate the pattern of glutamate-induced astrocyte calcium ([Ca(2+)](i)) increases through SERCA2b-mediated mechanisms.