Related Experiment Video
Updated: Apr 28, 2026

A Purification and In Vitro Activity Assay for a pppGpp Synthetase from Clostridium difficile
Published on: November 3, 2018
The estimation of pyrophosphate in urine with uridine-5'-diphosphoglucose pyrophosphorylase
Abstract:
The concentration of pyrophosphate in urine has been determined using the enzyme uridine-5'-diphosphoglucose pyrophosphorylase. The technique is relatively quick and easy to perform. Reproducibility of results was good, and results from recovery experiments were excellent. The mean concentration of pyrophosphate found in early morning urine samples from 14 normal adults was identical with that reported by other workers using an ion-exchange method to measure pyrophosphate. The pyrophosphate concentration was related approximately to the phosphate concentration.
Related Concept Videos
Phase II Reactions: Glucuronidation
Drug Dosing in Renal Diseases: Measurement of Glomerular Filtration Rate
Urine Studies I: Urinalysis
Imaging Studies V: Intravenous Urography and Retrograde Pyelography
Biosynthesis of Nucleic Acids

