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[Antitumor effect of tumor necrosis factor alpha and interferon gamma on human lung adenocarcinoma A549 cell line.]
1Department of Respiratory Medicine , The First Affiliated Hospital of Nanjing Medical University , Nanjing 210029 , P. R. China.
Background:
To explore the synergistic antitumor effect of tumor necrosis factor alpha (TNF-alpha) and interferon gamma ( IFN-gamma) on human lung adenocarcinoma A549 cell line.
Methods:
Cell growth inhibition was determined byMTT colorimetry assay.
Results:
Both TNF-alpha and IFN-gamma showed directly growth inhibition on human lung carcinoma A549 cell line. IFN-gamma could enhance cell growth inhibition effect of TNF-alpha when used together.
Conclusions:
TNF-alpha and IFN-gamma displayed significant synergistic antitumor effect on human lung adenocarcinoma A549 cell line. These results give experimental evidence for the multimodality therapy of human lung cancer and other tumors.
