[A study on immunity enhancement of frozen human lung cancer cells in vitro.]
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Updated: Jun 8, 2026
Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices
Published on: May 9, 2018
1Department of Thoracic Surgery , General Hospital of Wulumuqi , Lanzhou Military District , Urumqi , Xinjiang 830000 , P. R. China.
Background:
To investigate the mechanisms of cryo-immunotherapeutic effect.
Methods:
After the mixed culture of human lung cancer cells and autologous lymphocytes was conducted in vitro ,lymphocyte transformation rate (LTR) , soluble interleukin-2 receptor (SIL-2R) and interleukin-6 ( IL-6) in the supernatants of the frozen lung cancer cells and the control were detected. Meanwhile , the effects of the supernatants of the culture of frozen lung cancer cells and the control on the proliferation of autologous lung cancer cells were also determined with FCM measurement.
Results:
(1)The levels of SIL-2R , IL-6 and LTR in the group of frozen lung cancer cells were all highly significant higher than those in the group of the control ( P < 0. 01) . (2)The supernatant of the culture of the frozen lung cancer cells could remarkably inhibit the proliferation of the autologous lung cancer cells ( P < 0. 01) .
Conclusions:
(1)Frozen lung cancer cells may improve the immune function of lymphocytes through promoting their activation , proliferation and secretion of cytokines. (2)The activation of immune competent cells by freezing can remarkably inhibit the proliferation of lung cancer cells. Therefore , freezing can irritate the body's anti-tumor immunity.
