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Updated: Jun 8, 2026

Extracellular Glucose Depletion as an Indirect Measure of Glucose Uptake in Cells and Tissues Ex Vivo
Published on: April 6, 2022
Dose-dependent increase and decrease in active glucose uptake in jejunal epithelium of broilers after acute exposure
Agha Waqar Yunus1, Wageha A Awad, Susan Kröger
1Institute of Animal Nutrition, Department of Veterinary Public Health and Food Science, University of Veterinary Medicine Vienna, Vienna, Austria.
Chicken jejunal tissues show dose-dependent responses to ethanol. Low ethanol levels enhance glucose absorption, while higher levels inhibit it, indicating sensitivity in avian intestinal epithelia.
Area of Science:
- Animal Physiology
- Gastroenterology
- Pharmacology
Background:
- Limited data exists on ethanol's impact on avian gastrointestinal systems.
- Understanding ethanol's effects on chicken jejunum is crucial for poultry science.
Purpose of the Study:
- To investigate the electrophysiological effects of varying ethanol concentrations on chicken jejunal epithelium.
- To determine the dose-dependent impact of ethanol on active glucose absorption.
Main Methods:
- Utilized Ussing chambers to expose broiler jejunal tissues to different ethanol levels (0, 0.1%, 0.33%, 0.66%).
- Monitored electrophysiological variables like short-circuit current (Isc) and potential difference (Vt).
- Assessed responses to glucose and carbamoylcholine stimulation.
Main Results:
- Low ethanol (0.1%) significantly increased glucose-induced Isc and Vt, suggesting enhanced active glucose absorption.
- Higher ethanol concentrations (0.33%, 0.66%) significantly decreased basal and glucose-induced Isc and Vt.
- Ethanol did not affect carbamoylcholine-induced responses or tissue conductivity.
Conclusions:
- Chicken intestinal epithelia exhibit a dose-dependent sensitivity to ethanol.
- Ethanol can modulate active glucose absorption in chickens, with low levels enhancing and higher levels inhibiting it.
- This study provides the first evidence of ethanol's dual effect on avian intestinal glucose transport.
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