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[Comparison of refractometric and ultraviolet detection methods in lactulose assay by high performance liquid
C Vaccher1, P Berthelot, N Flouquet
1Laboratoire de Pharmacie Chimique, Université de Lille II.
Annales Pharmaceutiques Francaises
|January 1, 1990
Abstract:
Dosage of lactulose and determination of related sugars (lactose, fructose, galactose, épilactose) were undertaken by high performance liquid chromatography (HPLC). The present note described two modes of detection used simultaneously and compared: refractive index detector (RI) and multiwavelength ultraviolet detector (UV). HPLC is performed with an aminopropylsylil phase as stationary phase and an isocratic mobile phase containing acetonitrile and aqueous sodium phosphate. The results are outlined and discussed.