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Related Experiment Videos

Characterization of a Streptococcus mutans serotype e plasmid pJEB110.

J E Bergmann1, E Johanna, S Oloff

  • 1University of Hamburg Dental School, F.R.G.

Archives of Oral Biology
|January 1, 1990
PubMed
Summary

Researchers identified a novel 5.7 kb plasmid, pJEB110, in a mutans streptococcal strain from dental plaque. This plasmid is closely related to others found in serotype e strains.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Dental Plaque Research

Background:

  • Extrachromosomal elements like plasmids play crucial roles in bacterial adaptation and virulence.
  • Mutans streptococci are key pathogens in dental caries, and understanding their genetic elements is vital.
  • Previous studies have characterized various plasmids in different serotypes of Streptococcus mutans.

Purpose of the Study:

  • To investigate the presence and characteristics of extrachromosomal elements in mutans streptococcal strains.
  • To identify and analyze novel plasmids within dental plaque isolates.
  • To compare the genetic relatedness of newly discovered plasmids to known Streptococcus mutans plasmids.

Main Methods:

  • Screening of 300 mutans streptococcal strains isolated from dental plaque for extrachromosomal DNA.

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  • Plasmid DNA isolation and characterization, including size determination (5.7 kb for pJEB110).
  • Protein analysis using electrophoresis to identify strain-specific markers.
  • Restriction enzyme site mapping for comparative genetic analysis of plasmids.

    • Main Results:

    • One novel 5.7 kb plasmid, designated pJEB110, was identified in a serotype e Streptococcus mutans strain (JEB110).
    • The presence of pJEB110 correlated with a distinct 70 kDa protein band in electrophoretograms, differentiating it from plasmid-free and other plasmid-carrying strains.
    • Restriction mapping revealed pJEB110 is more closely related to pAM7 (from a serotype e strain LM7) than to plasmids from serotype c or f strains.

    Conclusions:

    • A new plasmid, pJEB110, has been discovered in a dental plaque-derived mutans streptococcal strain.
    • pJEB110 shares significant genetic similarity with other plasmids found in serotype e strains, suggesting potential common origins or functions.
    • The findings contribute to the understanding of plasmid diversity and evolution within Streptococcus mutans populations.