Cloning of Bacteroides gingivalis surface antigens involved in adherence

Despite the acknowledge importance of Bacteroides gingivalis as a periodontal pathogen, relatively little is known about the molecular basis for its pathogenicity. Recombinant DNA technology has created the opportunity to identify and characterize the virulence determinants. A Bact. gingivalis library was prepared in Escherichia coli JM83 with the vector pUC18. The library was screened for surface antigens by colony immunoblot and 337 putative surface antigen recombinants were identified. Two of these clones were found to have haemagglutinating activity. Antiserum raised against one of these clones reacted with a 43 kDa Bact. gingivalis protein that has haemagglutinating activity.