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Related Experiment Video

Updated: Jun 8, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Interactive proteomics research technologies: recent applications and advances.

Julia Petschnigg¹, Jamie Snider, Igor Stagljar

¹Department of Biochemistry & Department of Molecular Genetics, Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario, Canada.

Current Opinion in Biotechnology

|October 2, 2010

Summary

This summary is machine-generated.

Understanding protein-protein interactions is key to molecular biology and disease research. This study reviews the latest technologies for mapping these crucial protein complexes, known as interactomes.

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Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

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Last Updated: Jun 8, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

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Published on: November 15, 2017

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

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Published on: November 13, 2021

Area of Science:

Molecular Biology
Proteomics
Systems Biology

Background:

Proteins function within multiprotein complexes, essential for all biological processes.
Investigating protein-protein interactions (PPIs) and interactomes is vital for understanding molecular mechanisms and diseases.
Dissecting protein complexes aids in defining molecular functions and identifying drug targets.

Purpose of the Study:

To provide an overview of recent advancements in interactive proteomics research technologies.
To highlight the applications of these technologies in generating large-scale interaction datasets.
To emphasize the importance of studying protein complexes for biological insights.

Main Methods:

Review of current protein-protein interaction detection methods.
Focus on technologies adapted for large-scale interactome mapping.
Discussion of recent progress in interactive proteomics.

Main Results:

Significant progress has been made in adapting proteomics tools for large-scale interaction datasets.
Various methods are available for unraveling protein interaction networks.
Interactive proteomics technologies are rapidly evolving.

Conclusions:

Interactive proteomics is crucial for deciphering protein complex functions.
Advances in technology enable comprehensive interactome mapping.
Understanding protein interactions is fundamental for molecular biology and disease mechanism elucidation.