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Association between the nucleolus and the coiled body.
I Raska1, R L Ochs, L E Andrade
1W.M. Keck Autoimmune Disease Center, Scripps Clinic and Research Foundation, La Jolla, California 92037.
Journal of Structural Biology
|July 1, 1990
Summary
This study identifies p80-coilin in coiled bodies within mammalian cells and rat neurons. Coiled bodies associate with nucleoli in neurons, especially after drug treatment, suggesting a unique relationship.
Area of Science:
- Cell Biology
- Molecular Biology
- Neuroscience
Background:
- Coiled bodies are nuclear structures involved in RNA processing.
- p80-coilin is a specific marker for coiled bodies.
- The relationship between coiled bodies and nucleoli is not fully understood.
Purpose of the Study:
- To localize p80-coilin in mammalian cells and primary rat neurons.
- To investigate the association between coiled bodies and nucleoli.
- To examine how drug-induced nucleolar stress affects coiled body localization.
Main Methods:
- Light and electron microscopic immunocytochemistry to detect p80-coilin.
- Silver staining for nucleolar organizer regions.
- Treatment of cells with actinomycin D and 5,6-dichloro-1-beta-D-ribo furanosyl-benzimidazole.
Main Results:
- p80-coilin was localized in nuclear bodies containing fibrillarin in both cell types.
- Coiled bodies were frequently associated with nucleoli in rat neurons, unlike cycling cells.
- Drug treatments induced nucleolar changes and increased association of p80-coilin structures with nucleoli.
Conclusions:
- p80-coilin is a reliable marker for coiled bodies.
- Coiled bodies show a notable association with nucleoli in rat neurons.
- This association may be enhanced under conditions of nucleolar stress.