Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
UV–Vis Spectroscopy: Beer–Lambert Law01:09

UV–Vis Spectroscopy: Beer–Lambert Law

The Beer-Lambert law describes the relationship between absorbance and concentration, which combines the principles established by scientists Johann Heinrich Lambert and August Beer. Lambert's law states that when light passes through a medium, the loss in intensity is directly proportional to the original intensity and the path length of the light. Beer's law proposed that the transmittance of a solution remains constant if the product of concentration and path length is constant. The modern...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Deep-learning endomicroscope with large field-of-view and depth-of-field for real-time in vivo imaging of epithelial cancer hallmarks.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Hybrid endomicroscopic objective with monolithic multi-material achromatic triplet fabricated using two-photon lithography.

Applied optics·2026
Same author

Miniature 3D-printed rod-like refractive objective for endoscopic applications.

Journal of biomedical optics·2026
Same author

Snapshot hyperspectral imaging microscope enabled by cladded waveguide array fabricated with 2-photon additive manufacturing.

Biomedical optics express·2026
Same author

Dual-modality, deep-learning-enabled endomicroscope with large field-of-view and depth-of-field for real-time in vivo imaging of epithelial hallmarks of cancer.

bioRxiv : the preprint server for biology·2026
Same author

Fully 3D-printed endomicroscopic objective for two-photon, multi-wavelength excitation microscopy.

Biomedical optics express·2026
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Jun 8, 2026

Applying Hyperspectral Reflectance Imaging to Investigate the Palettes and the Techniques of Painters
07:05

Applying Hyperspectral Reflectance Imaging to Investigate the Palettes and the Techniques of Painters

Published on: June 18, 2021

Hyperspectral Shack-Hartmann test.

Gabriel C Birch1, Michael R Descour, Tomasz S Tkaczyk

  • 1College of Optical Sciences, University of Arizona, 1630 East University Boulevard, Tucson, Arizona 85721, USA. gcb@email.arizona.edu

Applied Optics
|October 2, 2010
PubMed
Summary
This summary is machine-generated.

A new hyperspectral Shack-Hartmann test bed accurately measures chromatic focal shift in miniature optics. This technology is crucial for developing advanced all-polymer endomicroscopes for broadband imaging.

More Related Videos

Hyperspectral Imaging as a Tool to Study Optical Anisotropy in Lanthanide-Based Molecular Single Crystals
07:24

Hyperspectral Imaging as a Tool to Study Optical Anisotropy in Lanthanide-Based Molecular Single Crystals

Published on: April 14, 2020

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Related Experiment Videos

Last Updated: Jun 8, 2026

Applying Hyperspectral Reflectance Imaging to Investigate the Palettes and the Techniques of Painters
07:05

Applying Hyperspectral Reflectance Imaging to Investigate the Palettes and the Techniques of Painters

Published on: June 18, 2021

Hyperspectral Imaging as a Tool to Study Optical Anisotropy in Lanthanide-Based Molecular Single Crystals
07:24

Hyperspectral Imaging as a Tool to Study Optical Anisotropy in Lanthanide-Based Molecular Single Crystals

Published on: April 14, 2020

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Area of Science:

  • Optical engineering
  • Microscopy instrumentation

Background:

  • Developing broadband achromatized all-polymer endomicroscopes requires precise characterization of miniature optics.
  • Chromatic focal shift (CFS) is a critical performance parameter for optical elements across a wide spectral range.

Purpose of the Study:

  • To develop and validate a hyperspectral Shack-Hartmann test bed for characterizing miniature optics.
  • To measure the chromatic focal shift (CFS) of glass singlet and achromatic lenses as benchmarks for polymer elements.

Main Methods:

  • A hyperspectral Shack-Hartmann test bed was constructed to measure optical performance.
  • Glass lenses were tested across the 500-700 nm spectral range in controlled steps.
  • Experimental results were compared with ZEMAX modeling predictions.

Main Results:

  • The hyperspectral Shack-Hartmann test bed demonstrated high accuracy, with experimental results closely matching ZEMAX model predictions.
  • Maximum errors were 12 micrometers for a singlet lens and 5 micrometers for an achromatic triplet lens.
  • The test bed effectively characterized CFS across the tested spectral range.

Conclusions:

  • The developed hyperspectral Shack-Hartmann test bed is a reliable tool for characterizing miniature optics.
  • This technology is essential for the advancement of broadband achromatized all-polymer endomicroscopes.
  • Future applications include aberration measurement and characterization of plastic endomicroscope components.