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A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target
Published on: May 10, 2022
Hepatitis B virus/hepatitis C virus upregulate angiopoietin-2 expression through mitogen-activated protein kinase
Yanmei Li1, Jizheng Chen, Chunchen Wu
1State Key Lab of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China Institute of Virology, University Hospital of Essen, Essen, Germany.
Aim:
To explore the molecular mechanism of hepatitis B virus (HBV)/hepatitis C virus (HCV) upregulate angiopoietin-2 (Ang-2) expression.
Methods:
Reverse transcription polymerase chain reaction (RT-PCR), quantitative real-time (qRT)-PCR and enzyme-linked immunosorbent assay (ELISA) analysis were used to measure the Ang-2 transcription and expression level. Reporter gene assays were used to determine the cis-element of the Ang-2 promoter. The specific inhibitors assay, immunofluorescence and western blot analysis were conducted to verify the signal pathway involved in the upregulation of Ang-2 expression.
Results:
The level of transcription and expression of Ang-2 increased in the HepG2.2.15 and Con-1 cells. Reporter gene assays in HepG2.2.15 and Con-1 cells revealed that HBV/HCV could enhance Ang-2 promoter expression by activating AP-1 and Ets1. Analysis with specific inhibitors indicated that HBV/HCV upregulated the expression of Ang-2 through mitogen-activated protein kinase (MAPK) pathways.
Conclusion:
This study illustrates a distinct mechanism by which a tumor virus modulates vasculature to promote tumorigenesis.
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